. Author manuscript; available in PMC: 2022 Oct 1.

Published in final edited form as: J Neurosci Res. 2021 Jul 2;99(10):2625–2645. doi: 10.1002/jnr.24864

TABLE 4.

Circadian parameters in Six3^syn female mice

	LD12:12		DD		Interaction (p)	Light main effect (p)	Genotype main effect (p)
	Six3^fl/fl (n = 8) Avg ± SEM	Six3^syn (n = 13) Avg ± SEM	Six3^fl/fl (n = 8) Avg ± SEM	Six3^syn (n = 13) Avg ± SEM	Interaction (p)	Light main effect (p)	Genotype main effect (p)
Period (hr)	23.98 ± 0.02	24.01 ± 0.01	23.63 ± 0.11	23.66 ± 0.10	F(1,19) = 0.14 (p = 0.71)	F(1,19) = 7.93 (p = 0.01)	F(1,19) = 0.001 (p = 0.98)

Alpha (hr)	11.43 ± 0.34	12.05 ± 0.39	12.05 ± 0.80	10.94 ± 0.61	F(1,19) = 3.02 (p = 0.10)	F(1,19) = 0.64 (p = 0.43)	F(1,19) = 0.74 (p = 0.40)

χ² amplitude (Qp)	2,590.35 ± 332.03	195.19 ± 158.17	2,683.01 ± 270.66	991.54 ± 160.62	F(1,19) = 7.21 (p = 0.01)	F(1,19) = 16.22 (p = 0.0003)	F(1,19) = 10.78 (p = 0.004)

Activity profile amplitude	N/A	N/A	96.62 ± 24.75	33.09 ± 16.17	N/A	N/A	t(16) = 2.15 (p = 0.047)

Note: Analysis of circadian parameters of Six3^fl/fl and Six3^syn females on running wheels. Wheel-running behavior was analyzed in LD12:12 and constant darkness (DD), with mean and standard error (SEM). Period, alpha, and χ² amplitude were analyzed via two-way repeated measures ANOVA and activity profile amplitude was analyzed via t test. Bolded values indicate significant differences, p < 0.05.