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Experiments where (A) optimal ratio of gRNA to CRISPR/Cas protein required for precomplexing will be determined, (B) the optimal amount of recombinant CRISPR/Cas will be determined (if we assume the optimal ratio will be 1:1). All experiments will be performed in duplicate, 4 gRNAs will be used for RNP formation (AAVS1, CDK4 and HPRT1 and non-targeting control gRNA).
