Table 7. Glucose, sucrose and mannitol concentrations optimization for GSM protein transduction.

Well	Glucose, mM	Sucrose, mM	Mannitol, mM
1	200	200	200
2	200	200	400
3	200	200	600
4	200	400	200
5	200	400	400
6	200	400	600
7	200	600	200
8	200	600	400
9	200	600	600
10	400	200	200
11	400	200	400
12	400	200	600
13	400	400	200
14	400	400	400
15	400	400	600
16	400	600	200
17	400	600	400
18	400	600	600
19	600	200	200
20	600	200	400
21	600	200	600
22	600	400	200
23	600	400	400
24	600	400	600
25	600	600	200
26	600	600	400
27	600	600	600

A mock transduction (i.e., GSM transduction mixture only, 27 variants according to Table 7) and non-treated control will be included to understand CRISPR/Cas RNP toxicity when compared to the non-treated sample.