Table 8. Sucrose and PEG1000 concentrations optimization during standard osmotic lysis of pinocytic vesicles technique (48-well transduction plate layout).
| Sucrose | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Concentration in Complete transduction mixture, mM | |||||||||||
| 0 | 200 | 300 | 400 | 500 | 600 | ||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||||
| PEG1000 | Concentration in Complete transduction mixture, % | A | - | - | - | - | - | - | - | - | |
| 0 | B | - | - | ||||||||
| 5 | C | - | - | ||||||||
| 10 | D | - | * | - | |||||||
| 20 | E | - | - | ||||||||
| F | - | - | - | - | - | - | - | - | |||
* - optimal combination according to [65]. Rows “A”, “F” and columns #1, #8 will be filled with PBS to avoid edge effect.