Fig 3. RORγt-mediated inhibition of IL-17A production in human Th17 cell cultures.

Schematic of human Th17 differentiation (A) or Th17 maintenance (B) assays. Representative flow cytometry plots of intracellular cytokine staining from cultures from (A) including Th0 cell nonpolarizing condition (C). IL-17A cytokine production from enriched naïve CD4⁺ T cells from healthy donor PBMC cultured under Th17 cell conditions for 6 days (D) or from enriched human Th17 cells from healthy donor PBMC cultured with IL-23 and IL-1β, for 4 days (E), in the presence of the indicated RORγt inhibitors Compounds 1–3. Data are normalized to and represented as percent inhibition compared to DMSO controls. Error bars are representative of 4 individual donors, from 2 independent experiments.