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. 2021 Oct 28;17(10):e1009862. doi: 10.1371/journal.pgen.1009862

Fig 4. The regulation of ZBED6 in the muscle.

Fig 4

(n = 3). Gastrocnemius muscle (GM), longissimus dorsi (LD). (A) PCA of the expressed genes of multiple tissues in WT and ZBED6-/- pigs. (B) Number of DEGs in heart, GM and LD in ZBED6-/- pigs. (C) Overlap DEGs in heart, GM and LD. (D) Heatmap of common 14 DEGs in heart, GM and LD. 35520 and 31306 are two ensemble ID, ENSSSCG00000035520 and ENSSSCG00000031306. The genes in red type contain at least one ZBED6 ChIP-seq peak. (E) qPCR analysis of CDKN1A mRNA in the heart, GM and LD from ZBED6-/- pigs. (F) ChIP-PCR analysis in gastrocnemius muscle of ZBED6 occupancy at the binding sites of IGF2, CDKN1A and GNAZ. The results are the means ± SEMs. *p < 0.05; **p < 0.01, *** p < 0.001,Student’s t test.