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. 2021 Oct 28;17(10):e1009878. doi: 10.1371/journal.pgen.1009878

Fig 6. Spot 42 regulates the tuning of gal protein levels towards an anabolic pathway.

Fig 6

A) Western blot analysis of the protein products of gal operon (GalE, GalT, GalK and GalM) from WT and Δspf cells. B) Physiological effect of Spot 42 deletion. This was measured by comparing growth rate of MG1655 and MG1655Δspf cells in various media: LB with 0.5% galactose (light blue and green), M9 with 0.2% glucose (grey and yellow) and M9 with 0.5% galactose (blue and red). The growth of MG1655Δspf cells was identical to that of MG1655 in LB-galactose and M9-glucose media. In M9-galactose medium, MG1655Δspf cells (red) showed slower growth than that of MG1655 cells (blue). Error bars represents the mean fold-change ± standard deviation of three replicates from three independent experiments (n = 3).