Table 1.
Biophysics and Pharmacology of mitoBK and nBK.
| Tissue/organism | Methodology | Unitary conductance (pS) and conditions | Biophysical parameters | Pharmacology | References |
|---|---|---|---|---|---|
| Potato tuber (mitoBK) | Lipid bilayer | 615±12 – gradient cis/trans 50/450mMK+ | Vr =+34mV, P0 =0.49 (0 Ca2+; 0mV)/0.84 (300μM Ca2+; 0mV) | NS1619 Iberiotoxin |
Koszela-Piotrowska et al., 2009 |
| Rat brain (mitoBK) | Lipid bilayer | ~565 – gradient cis/trans 200/50mMK+ | Vr =−30mV | Iberiotoxin charybdotoxin | Fahanik-Babaei et al., 2011b; Singh et al., 2012 |
| Drosophila melanogaster (mitoBK) | Single channel in mitoplast | 382±8 – symmetric 150mMK+ | N/A | NS1619 Paxilline |
Gururaja et al., 2019 |
| Guinea pig cardiomyocytes (mitoBK) | Single channel in mitoplast | 307±4.6 – symmetric 150mMK+ | N/A | NS1619 NS11021 Paxilline |
Xu et al., 2002; Aon et al., 2010 |
| Rat cardiomyocites (mitoBK) | Single channel in mitoplast | 303±19 – symmetric 140mMK+ | V1/2 =−55mV (12μM Ca2+) | diCl-DHAA paxilline | Sakamoto et al., 2008; Balderas et al., 2019 |
| Rat astrocytes (mitoBK) | Single channel in mitoplast | 296±18 – symmetric 150mMK+ | N/A | CGS7181 CGS7184 paxilline charybdotoxin | Cheng et al., 2008; Augustynek et al., 2018 |
| Human glioma (mitoBK) | Single channel in mitoplast | 295±18 – symmetric 150mMK+ | Vr =+70mV (150mM Na+), EC50(Ca2+)=900nM (+60mV)/6.9μM (−20mV) | Charybdotoxin | Siemen et al., 1999 |
| Rat non-neoplastic astrocytes (mitoBK) | Single channel in mitoplast | ~290 – symmetric 140mMK+ | N/A | Iberiotoxin | Cheng et al., 2011 |
| HEK293T (DEC splice variant; mitoBK) | Single channel in mitoplast | 290±3 – symmetric 150mMK+ | V1/2 =+19.2mV (100μM Ca2+), t0 =10.2ms (+60mV) | Paxilline | Gałecka et al., 2021 |
| Human glioma (mitoBK) | Single channel in mitoplast | 276±9 – symmetric 150mMK+ | Vr =+9.3±2.4mV; O2: 21.1±1.2nmol/ml | Charybdotoxin | Gu et al., 2007 |
| Rat ventricular myocytes (mitoBK) | Single channel in mitoplast | ~270 – symmetric 140mMK+ | N/A | 17B-estradiol paxilline | Ohya et al., 2005 |
| Human endothelium (mitoBK) | Single channel in mitoplast | 270±10 – symmetric 150mMK+ | t0 =0ms (−60mV)/~70ms (+60mV) | NS1619 NS11021 paxilline iberiotoxin | Bednarczyk et al., 2013a |
| Rat brain (mitoBK) | Lipid bilayer | 265±5 – gradient cis/trans 50/450mMK+ | Vr =+50mV, Po (+70mV)=0.5 (0 Ca2+)/0.77 (300μM Ca2+) | NS1619 iberiotoxin charybdotoxin | Skalska et al., 2009 |
| Human glioma (mitoBK) | Single channel in mitoplast | 262±12 – symmetric 150mMK+ | N/A | Charybdotoxin | Walewska et al., 2018 |
| Dictyostelium discoideum (mitoBK) | Lipid bilayer | 258±12 – gradient cis/trans 50/150mMK+ | Vr =+27.6±0.5mV, P0 =0.14 (1μM Ca2+; 0mV)/0.48 (100μM Ca2+; 0mV) | NS1619 NS11021 paxilline iberiotoxin | Laskowski et al., 2015 |
| Rat hippocampal neurons (nBK) | Single channel in nuclear envelope | 217 pS – symmetric 135mMK+ | Vr =0mV, P0 =0.3 (5μM Ca2+)/0.78 (10μM Ca2+) | Paxilline | Li et al., 2014 |
| Rat brain (mitoBK) | Lipid bilayer | ~211 – gradient cis/trans 200/50mMK+ | Vr =−30mV | Iberiotoxin | Fahanik-Babaei et al., 2011a; Singh et al., 2012 |
| Rat pancreas (nBK) | Single channel in nuclear envelope | 200±25 pS – symmetric 148mMK+ | Vr =0mV | N/A | Maruyama et al., 1995 |
| Mice cardiomyocytes (mitoBK) | Single channel in mitoplast | ~190 – internal/external=130:10mMK+ | t0 =9.23ms, P0 =0.79 (+80mV) | NS11021 Paxilline |
Soltysinska et al., 2014 |
| Mice cardiomyocytes (mitoBK) | Single channel in mitoplast | ~145 – symmetric 150mMK+ | P0 =0.28 (1μM Ca2+); 0.54 (100μM Ca2+) | NS11021 Paxilline |
Frankenreiter et al., 2017 |