FIG. 4.

Temporal expression pattern of the phy-1 (□), phy-2 (⧫), and pdi-2 (┘) transcripts during postembryonic development. A semiquantitative reverse transcriptase PCR approach was applied to examine the ratio of expression (y axes) of the individual enzyme-encoding genes to that of the constitutively expressed gene ama-1. The arbitrary values for phy-1 and phy-2 were plotted together for comparison. All values were obtained from mRNA of synchronously maintained larval and early adult stages at 25°C. L1 to L4, first to fourth larval stages.