Figure 4.

scRNA-seq analysis reveals an adult Ngn3+ population able to differentiate into endocrine cells

(A) scRNA-seq experimental design.

(B) UMAP projection of 21,813 pancreatic islet cells. Cells are colored by assigned cluster.

(C) Expression of α (Gcg, Arx, and Irx2), β (Ins1, Nkx6-1, and Ucn3), δ (Sst and Rbp4), ductal (Krt19), acinar (Pnlip), endothelial (Pecam1), and immune (Cd74) cell markers across the scRNA-seq dataset.

(D) Violin plots for expression of Ins1, Gcg, Sst, and Krt19 across identified clusters.

(E) UMAP projection of Ngn3 (Neurog3) and tdTomato expression across a single-cell dataset.

(F) Violin plots for expression of Ngn3 in Ngn3+ cells (left) and tdTomato in tdTomato+ cells (right) across the identified clusters.

(G) Expression heatmap of α, β, δ, and ductal cells markers across Ngn3+ and tdTomato+ cells.

(H) Monocle 3.12 pseudotime analysis of Ngn3+ and tdTomato+ cells (left, predicted lineage trajectories; right, pseudotime projection in the predicted trajectories).

See also Figure S3.