REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-C-peptide	Cell Signaling	4593 (RRID: AB_10691857)
Goat anti-GFP	Abcam	ab6673 (RRID: AB_305643)
Mouse anti-Insulin	Sigma-Aldrich	I2018 (RRID: AB_260137)
Rabbit anti-RFP	TebuBio	600-401-379 (RRID: AB_2209751)
Rat-anti-Somatostatin	Abcam	ab30788 (RRID: AB_778010)
Goat anti-TdTomato	Biorbyt	Orb182397 (RRID: AB_2687917)
Mouse anti-Urocortin3	Phoenix Pharmaceuticals	Cat# H-019-29
Alexa Fluor 488 donkey anti-mouse	Thermo	A-21202 (RRID: AB_141607)
Alexa Fluor 546 donkey anti-rabbit	Thermo	A10040 (RRID: AB_2534016)
Alexa Fluor 647 goat anti-rat	Thermo	A-21247 (RRID: AB_141778)
Alexa Fluor 488 goat anti-goat	Thermo	A-11055 (RRID: AB_2534102)
Chemicals, peptides, and recombinant proteins
Fluorescein labeled Dolichos Biflorus Agglutinin (DBA)	Vector labs	FL-1031
Tamoxifen	Sigma	T5648
Peanut oil	Sigma	P2144
Formalin solution, neutral buffered, 10%	Sigma	HT501128
Bovine Serum Albumin	Sigma	A7906
Trisodium citrate dihydrate	Sigma	S1804
Methyl salicylate	Sigma	M6752
Triton X-100	Sigma	T9284
Sudan Black B	Sigma	199664
Methanol	Sigma	32213
Histo-Clear	Fisher	12358637
Deposited data
scRNA-seq of islet cells from Ngn3-CreERT; R26-CAG-tdTomato mice	This paper	GEO: GSE138038
Experimental models: Organisms/strains
Mouse: Hnf1bTg(Hnf1b-cre/ERT2)1Jfer	Solar et al., 2009	(RRID: IMSR_JAX:027681)
Mouse: (Tg)(Ins1-cre/ERT)1Lphi	The Jackson Laboratory	Jax: 024709 (RRID: IMSR_JAX:024709)
Mouse: Tg(Neurog3-cre/Esr1∗)1Dam	The Jackson Laboratory	Jax: 008119 (RRID: IMSR_JAX:008119)
Mouse: C57BL/6-Ins2Akita/J	The Jackson Laboratory	Jax: 003548 (RRID: IMSR_JAX:003548)
Mouse: tm9.1(CAG-tdTomato)Hze	The Jackson Laboratory	Jax: 007909 (RRID: IMSR_JAX:007909)
Mouse: Neurog3tm1(EGFP)Khk	MMRRC	000344-UCD (RRID: MMRRC_000344-UCD)
Software and algorithms
StrataQuest Analysis Software	Tissue Gnostics	https://tissuegnostics.com/en/products/analysing-software/strataquest
Zen 2.3	Zeiss	https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html
Imaris 8.3.1	Bitplane	https://imaris.oxinst.com/packages
Cell Ranger 3.2.0	10X Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation
RStudio 3.6.1	RStudio	https://www.rstudio.com/
Prism 7 for MacOS	Graphpad	https://www.graphpad.com/scientific-software/prism/
FastQC	Andrews, 2010	https://www.bioinformatics.babraham.ac.uk/projects/fastqc
Cutadapt	Martin, 2011	https://github.com/marcelm/cutadapt
Scater	McCarthy et al., 2017	https://bioconductor.org/packages/release/bioc/html/scater.html
Seurat	Satija et al., 2015	https://satijalab.org/seurat/
TSCAN	Ji and Ji, 2016(Ji and Ji, 2016)	https://bioconductor.org/packages/release/bioc/html/TSCAN.html
SCE (Single Cell Experiment)	Amezquita et al., 2020(Amezquita et al., 2020)	https://bioconductor.org/packages/release/bioc/html/SingleCellExperiment.html