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. 2021 Nov 9;10:e71683. doi: 10.7554/eLife.71683

Figure 5. Probing the binding interface of N. meningitidis LbpB (NmLbpB) with lactoferrin.

Figure 5.

(A) A zoomed view of the N-lobe of NmLbpB along the lactoferrin (Lf) interaction interface, highlighting primary residues involved in binding. (B) Enzyme-linked immunosorbent assays (ELISAs) to test the effects of structure-guided mutations of NmLbpB on Lf binding along the interaction interface. (C) Analysis of the binding parameters using isothermal titration calorimetry analysis of wild type and mutants of NmLbpB measuring the effects on Lf binding.