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. 2021 Oct 11;10:e72833. doi: 10.7554/eLife.72833

Table 2. Plasmids used in this study.

Plasmid name Description Reference
pRS316 URA3-dependent CEN-based yeast-E. coli shuffle plasmid AmpR Sikorski and Hieter, 1989
pRS416 URA3-dependent CEN-based yeast-E. coli shuffle plasmid AmpR Geissler et al., 1996
pAFS125 GFP-TUB1-URA3-containing integration plasmid Straight et al., 1997
pSM1027 GFP-TUB1-ADE2-containing integration plasmid Caydasi et al., 2010b
pAK010 mCherry-TUB1-kıTRP1-containing integration plasmid Khmelinskii et al., 2007
pAK011 mCherry-TUB1-URA3-containing integration plasmid Khmelinskii et al., 2007
pBK067 mCherry-TUB1-LEU2-containing integration plasmid Caydasi et al., 2014
pWS103-1 pRS304-Gal1-UPL-TEM1 Shou et al., 1999
pMM5 p423-Gal1-lexA-Myc Geissler et al., 1996
pMM6 p425-Gal1-Gal4-HA. Geissler et al., 1996
pCDV471 pEG202 ADH1-LexA-DBD-GLC7 Lenssen et al., 2005
pEG202 2 μm ADH1-LexA-DBD-HIS3 Pereira et al., 1999
pCL2-1 pMM6-BUB2 Höfken and Schiebel, 2004
pCL4a-3 pMM6-BFA1 Höfken and Schiebel, 2004
pHA132-2 pMM6-KIN4 (1–750 aa) Gift from G. Pereira
pHA69-1 pMM5-BUD14 Gift from G. Pereira
pHA70-1 pMM6-BUD14 Gift from G. Pereira
pTH221 pMM6-KEL Höfken and Schiebel, 2004
IKY006 pMM5-BUD14-5A This Study
IKY007 pMM5-BUD14-F379A This Study
IKY008 pMM5-BUD14-∆SH3 This Study
IKY010 pMM6-BUD14-5A This Study
IKY011 pMM6-BUD14-F379A This Study
IKY012 pMM6-BUD14-∆SH3 This Study
pMK125 pRS416 ADH-BUD14 Knaus et al., 2005
pMK131 pRS416 ADH-BUD14-F379A Knaus et al., 2005
pMK60 pRS416-endogenous BUD14-promoter-GFP-BUD14 Knaus et al., 2005
pDKY001 pRS416-endogenous BUD14-promoter-GFP-BUD145A (135A 137A 138A 139A 140A) This Study
pDKY003 pRS416-endogenous BUD14-promoter-GFP-BUD14∆SH3(∆aa 262–318) This Study
pHK002 pRS416-endogenous BUD14-promoter-GFP-Bud14-F379A This study
pGW399-1 pRS316-KAR9 Caydasi et al., 2010b
pSM805 pRS305-pGal1 König et al., 2010
pHK003 pSM805 pGal1-Bud14-Leu2 This study
pHK004 pSM805 pGal1-Bud14-F379A-Leu2 This study
pIK007 pMM5-Bud14-F379A This study
pSM903-4 pRS316-LTE1 Höfken and Schiebel, 2002
pSM908 pRS316-DBF2 Caydasi et al., 2017
pSM926 pRS316-MOB1 Caydasi et al., 2017
pUG120 pRS316-CDC14 Gruneberg et al., 2000
pBS9 pRS316-CDC15 Caydasi et al., 2017
pCL33 pRS316-CDC5 Höfken and Schiebel, 2004