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. 2022 Feb;192(2):195–207. doi: 10.1016/j.ajpath.2021.10.009

Figure 4.

Figure 4

In situ hybridization (ISH) and immunohistochemistry findings in lung. RNAScope in situ hybridization showed widespread, punctate staining in the lungs at 2 and 4 days post inoculation (DPI), but was present only in a small focus within one animal at 7 DPI. No staining was present at 14 DPI (RNAScope in situ hybridization with red chromogen and hematoxylin counterstain). Immunostaining to detect Iba-1 revealed that macrophages comprised a major portion of the inflammatory infiltrate beginning at 2 DPI and peaking at 7 DPI. CD3+ lymphocytes represented a much smaller portion of the inflammatory population in the lungs but also increased from 2 to 7 DPI and then decreased at 14 DPI. (Immunostaining was performed with brown chromogen and hematoxylin counterstain.) Original magnification, ×20.