Figure 3.

TIPs inhibit SARS-CoV-2 in donor-derived lung organoids

(A) Schematic of primary human small-airway epithelial cell organoids.

(B) Bright-field micrograph of organoids at day 2 following establishment from one representative donor. Scale bar, 150 μm.

(C) Viral transcripts in SARS-CoV-2-infected (MOI = 0.5) lung organoids transfected with Ctrl, TIP1, or TIP2 RNA assayed by qRT-PCR to E gene at 24 h post-infection.

(D) Viral titer quantification by plaque assay (PFU/mL) for samples in (C).

For all panels: ^∗∗∗p < 0.001, ^∗∗p < 0.01, ^∗p < 0.05 from Student’s t test. See also Figure S2.