Figure S2.

Expression analysis of inflammatory pathways in TIP-treated cells, TIP post-infection activity, and culturing of lung organoids from primary human small-airway epithelial cells, related to Figures 2 and 3

(a) Analysis of relative expression (by qRT-PCR) of a panel of inflammatory genes based on (Nelson et al., 2020) in TIP RNA and Ctrl RNA transfected Vero cells in the absence of SARS-CoV-2 infection. No significant overexpression of inflammatory pathways, interferon-stimulated genes, or RNA sensors. (b) Efficacy of TIP in post-infection setting in cell culture. Cells were infected at MOI = 0.05, transfected with TIP or Ctrl RNA at 8hrs or 16hrs post-infection, and harvested at 48hrs post-infection. Viral RNA copies were quantified by qRT-PCR using N gene primers (normalized to GAPDH). (c) Transmission micrographs of human small airway epithelial cells from three donors cultured in matrigel in the presence of HAO (human airway organoid) medium. Organoids were monitored under transmission microscope at 10x magnification (size bar: 300 μm). Representative images shown for days 4, 8 and 12. [For all panels: ns denotes not significant, ^∗ denotes p < 0.05 from Student’s t test].