Figure S6.

Histopathology imaging of Syrian hamster lungs following pre- and post-infection treatment, related to Figure 7

(a) Animals were treated intranasally with TIP or Ctrl RNA LNPs 6 hr prior to and 18hrs following SARS-CoV-2 infection (10⁶ PFU). Lungs were harvested at 5 days post-infection and histopathology was performed. Micrographs of brightfield imaging of H&E-stained lung sections from all animals (top: Ctrl RNA treated hamsters; bottom: TIP-treated hamsters). Stitched images were analyzed using Leica Aperio ImageScope software. For each animal, whole lung shown on above and a representative zoomed-in section to visualize histopathology shown below. (b) Animals were infected with SARS-CoV-2 (10⁶ PFU) and, 12hrs later, were intranasally treated with either TIP or Ctrl RNA LNPs. Lungs were harvested at 5 days post-infection and histopathology performed. Micrographs of brightfield imaging of H&E-stained lung sections from all animals (top: Ctrl RNA treated hamsters; bottom: TIP-treated hamsters). Stitched images were analyzed using Leica Aperio ImageScope software. For each animal, whole lung shown on above and a representative zoomed-in section to visualize histopathology shown below. Scale bars are as indicated, n = 5 for each group. Labels that were added to the raw images during sectioning were covered during figure preparation, and size bars were added to the image.