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. 2000 Jun;20(11):4106–4114. doi: 10.1128/mcb.20.11.4106-4114.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 5 — Analysis of CX₃CR1 function in microglia. (A) Surface CX₃CR1 staining of microglial cells isolated via Percoll density gradient from collagenase-digested brains of wt, CX₃CR1^+/GFP, and CX₃CR1^GFP/GFP mice. Cells were stained for the indicated surface markers (CD11b and CX₃CR1) and gated according to scatter and viability as indicated. (B) Peripheral nerve transection experiment. Coronal section through operated and contralateral control facial nerve nuclei of axotomized CX₃CR1^+/GFP mouse day 7 after axotomy. Section were stained with an anti-neuronal nucleus-specific antibody (NeuN) and Cy5-coupled sheep anti-mouse IgG serum. (C) Quantitative evaluation of microglial reaction in response to facial nerve transection in operated CX₃CR1^+/GFP and CX₃CR1^GFP/GFP mice. The volume analyzed in the facial nerve nucleus cross sections represents about 0.25 mm² by 12 μm. Results are presented as means (± standard deviations) of 16 sections obtained from four mice of each genotype per time point.

FIG. 5 — Analysis of CX₃CR1 function in microglia. (A) Surface CX₃CR1 staining of microglial cells isolated via Percoll density gradient from collagenase-digested brains of wt, CX₃CR1^+/GFP, and CX₃CR1^GFP/GFP mice. Cells were stained for the indicated surface markers (CD11b and CX₃CR1) and gated according to scatter and viability as indicated. (B) Peripheral nerve transection experiment. Coronal section through operated and contralateral control facial nerve nuclei of axotomized CX₃CR1^+/GFP mouse day 7 after axotomy. Section were stained with an anti-neuronal nucleus-specific antibody (NeuN) and Cy5-coupled sheep anti-mouse IgG serum. (C) Quantitative evaluation of microglial reaction in response to facial nerve transection in operated CX₃CR1^+/GFP and CX₃CR1^GFP/GFP mice. The volume analyzed in the facial nerve nucleus cross sections represents about 0.25 mm² by 12 μm. Results are presented as means (± standard deviations) of 16 sections obtained from four mice of each genotype per time point.