Fig. 1.

Biphasic activation of COX-2 and L-PGDS in rat primary lung fibroblasts stimulated by LPS.(A) Rat primary lung fibroblasts were incubated with LPS (1 μg/ml) for 0, 6, 12, 24, 48, 72 h. (B–E) Relative protein levels of COX-2, L-PGDS, mPGES-1 and mPGES-2 were determined by western blot analysis. (F and G) Supernatants were collected at 0, 6, 12, 24, 48 and 72 h after LPS challenge for ELISA detection. PGE₂ and PGD₂ levels at each time point were determined by ELISA. Data are presented as mean ± SEM, n = 6 *p < 0.05, **p < 0.01,***p ＜ 0.001,****p < 0.0001.