Fig. 2.

PDX enhances the secondary peak of COX-2, L-PGDS via the ALX/FPR2 receptor in vitro.(A). Primary lung fibroblasts were incubated with LPS (1 μg/ml) for 48 h. Fibroblasts were treated with various concentrations of PDX (1 nM, 50 nM, 100 nM) for 24 h. BOC-2 (10 μM) was administered 30 min prior to PDX (100 nM) treatment, then cells were harvested and sonicated at 48 h. (B and C) The protein expression of COX-2 and L-PGDS after PDX treatment were tested by western blot and analyzed by densitometry compared to β-actin. (D and E) COX-2 and L-PGDS protein levels after the BOC-2 treatment were detected by western blot. Data are shown as mean ± SEM, n = 3–10. *p < 0.05, **p < 0.01,***p ＜ 0.001,****p < 0.0001.