Fig. 6.

PDX promotes the inflammatory resolution by enhancing the activation of ALX/FPR2 receptor and the COX-2-L-PGDS-PGD₂ expression in vivo.(A).SD rats were administered with LPS (3 mg/kg) or the same volume of sterile intravenously, PDX (5 μg/kg) were administered intravenously to rats at 12 h after LPS treatment, BOC-2 600 ng/kg was given for 1 h before PDX treatment. Lung tissues were collected at 24 h. (B) Representative pathological H&E staining sections of lung tissues. (Original magnification, 200x; inset, 400x). (C) Acute lung injury scores assessment of each group. (D–F) The mRNA expression level of Inflammatory cytokines: IL-1β, IL-6, TNF-α. (G and H) Expression levels of COX-2 and L-PGDS protein after PDX treatment was determined by western blot analysis. (I and J) Concertation of PGE₂ and PGD₂ were detected by ELISA. All data are presented as mean ± SEM, n = 6. *p < 0.05, **p < 0.01,***p ＜ c0.001,****p < 0.0001.