Figure 2.

N-terminusdetermines the extra processing and mitochondriallocalization of NSP3

A. Schematic illustration of chimeric plasmids used in this figure. B. The processing site of CoV2-NSP3 is localized at its N-terminus. Green arrow indicates the released EGFP. NO_ex indicates no exchange, PLpro_ex indicates the exchange of PLpro domain, whereas Ubl1–HVR_ex indicates the exchange of Ubl1 and HVR domains. Asterisks indicate non-specific signals. C. The N-terminus of CoV2-NSP3 re-locates CoV1-NSP3 to mitochondria. Scale bar, 10 μm. CANX, Calnexin (an ER marker); ER, endoplasmic reticulum.