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. 2021 Feb 20;35(6):1281–1290. doi: 10.1007/s10557-021-07151-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Outgrowth and characterisation of coronary endothelial outgrowth cells from atherothrombotic specimens. Outgrowth of coronary endothelial cells from dissected atherothrombotic specimen at 24 hours (a), colony formation at 7 days (b), and confluent cobblestone morphology at first passage (c). Cells stain positive for cytoplasmic expression of granular von Willebrand factor indicative of Weibel–Palade bodies (pink) and cell surface expression of CD31 (green) with 4',6-diamidino-2-phenylindole (DAPI) as a nuclei counterstain (blue) (d). Uptake of fluorescent labelled Dil-acetylated-LDL (red) with DAPI as a counterstain (e) and tube-like structure formation on Matrigel^TM(f). Scale bar 100 μm. Coronary endothelial outgrowth cells strongly express cell surface antigens CD146, CD31, CD105, and CD54 and cytoplasmic antigens von Willebrand factor (vWF) and endothelial nitric oxide synthase (eNOS), but do not express the pan leucocyte antigen CD45. Unstained (grey) and stained cells (black) (g)