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. 2021 Feb 20;35(6):1281–1290. doi: 10.1007/s10557-021-07151-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Coronary endothelial outgrowth cells do not incorporate into murine vessels in vivo. Immunohistochemistry of subcutaneously implanted sponges embedded with CEO cells (a–c), EOCs (d–f), and HUVECs (g–i). Sections were stained with antibodies cross-reactive to both mouse and human antigens (red) or specific to human antigens (green) using 4',6-diamidino-2-phenylindole (DAPI) as a counterstain. Auto-fluorescent erythrocytes are presence in vessel lumen demonstrates the vessels were contiguous with the host circulation. Scale bars 50 μm. In contrast to late endothelial outgrowth cells (EOCs) and human umbilical vein endothelial cells (HUVECs), coronary endothelial outgrowth (CEO) cells did not incorporate into murine vessel (j) (one-way analysis of variance P = 0.019; Bonferroni post-test *P < 0.05 for CEO cells versus HUVECs. n = 4–10)