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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Pharmacogenet Genomics. 2022 Jan 1;32(1):1–9. doi: 10.1097/FPC.0000000000000445

Fig. 3. Functional analysis of FAM114A2 in THP-1 cells.

Fig. 3

A) FAM114A2 siRNAs and scramble were transfected into THP-1 cells and untreated control, following which THP-1 cells were treated with PMA (150mM) for 24 hours to transform the cells to macrophages. Cell culture supernatant was collected for TNFα ELISA analysis. All TNFα level values are shown as relative to PMA treated scramble control. All experiments were conducted in triplicate.

B) Immunoblot analysis showing FAM114A2 gene knockdown increased the autophagy related protein, P62, and decreased the LC3 protein level - Representative Immunoblotting image shown.

C) Quantitative analysis of Immunoblotting results using Image J. All protein expression values were normalized to GAPDH and are shown relative to scramble control in each group. All experiments were conducted in triplicate.

phorbol 12-myristate 13-acetate – PMA, * p<0.05 ** p<0.01.