FIG. 3.

Expression of Ntan1 mRNA and localization of NTAN1p. (A) Whole-mount in situ hybridization of wild-type (wt) and Ntan1^−/− embryos (left four panels, light background) with an antisense RNA probe derived from a 0.3-kb fragment of the Ntan1 cDNA (nt 108 to 448) that was absent from the Ntan1^−/− allele. The regions of high Ntan1 expression in the tail buds (t), forelimb buds (fl), and hindlimb buds (hl) are indicated. The right two panels (dark background) show the results of in situ hybridization with an antisense (AS) Ubr1 cDNA probe. (B) Intracellular localization of mouse NTAN1p. NIH 3T3 cells were transiently transfected with a plasmid expressing the NTAN1p-GFP fusion (see Materials and Methods). Typical fluorescence patterns (a, c, and e) and the matching phase-contrast images (b, d, and f) are shown (see text).