Fig. 6. Dynamicity of the cortical actin array in WT, vln3, mpk3/6 guard cells induced by MAMP.

a Actin array remodeling in guard cells is shown in indicated genotypes treated with mock or flg22. Images were merged from three images with 1-min intervals colored in red, green and blue. A white color indicates actin structures that remain relatively stationary during this time period. Scale bar, 5 µm. b Correlation coefficient analysis was performed on time-lapse series from WT and mutant cells treated with mock or 10 µM flg22 for 10 min. The extent of actin rearrangements or the overall dynamicity of the actin array was determined by decay in correlation as the temporal interval increased. Lower correlation values correspond with higher dynamicity of the actin array. Error bars represents SEM. Analyses were performed on time-lapse series taken from 10 leaves for each treatment and genotype. The sample sizes are indicated. Different letters indicate significant differences at P < 0.05, as determined by two-way ANOVA with Tukey’s multiple comparisons test. Black or red letters indicate the data from mock or flg22 treatment, respectively. The exact p values are provided in the Source Data file.