Table I.
Genes significantly regulated in Tregs treated with PD-1 blockade versus untreated.i
| Upregulated | |||
|---|---|---|---|
| Gene | baseMean | Log2FoldChange | padj |
| Klf2 | 1351.561 | 1.473 | 5.55E-16 |
| Jun | 1034.796 | 0.984 | 1.86E-07 |
| Rhob | 300.810 | 1.230 | 2.31E-05 |
| Ubc | 5773.062 | 0.635 | 0.0004 |
| S1pr1 | 1512.735 | 0.629 | 0.0016 |
| Fos | 809.493 | 0.788 | 0.0016 |
| Ier2 | 646.815 | 0.774 | 0.0083 |
| Adrb2 | 328.347 | 0.830 | 0.0088 |
| Klf3 | 254.169 | 0.903 | 0.0104 |
| Atp1b1 | 72.096 | 1.011 | 0.0221 |
| Tsc22d3 | 942.382 | 0.746 | 0.0221 |
| Bcl2 | 203.146 | 0.940 | 0.0288 |
| Snord17 | 5426.186 | 0.630 | 0.0319 |
| Card6 | 481.202 | 0.731 | 0.0350 |
| Downregulated | |||
| Gene | baseMean | Log2FoldChange | padj |
| Arl5a | 5260.143 | −0.655 | 0.0003 |
| Sypl | 553.684 | −0.809 | 0.0016 |
| Ccr5 | 652.223 | −0.732 | 0.0016 |
| Ltf | 48.023 | −1.069 | 0.0092 |
| Itgb8 | 972.937 | −0.662 | 0.0152 |
| Gcnt1 | 300.171 | −0.797 | 0.0220 |
| Klrg1 | 370.393 | −0.712 | 0.0499 |
i
Foxp3-GFP mice were injected with 1 × 104 T11 (claudin-low) cells and were untreated or treated with 200μg α-PD1 antibody (J43) injected IP twice a week. Tumors were harvested at 150 mm2, digested, enriched for lymphocytes, and GFP+ Tregs were sorted to greater than 90% purity using MoFlo XDP cell sorter. RNA was isolated from sorted cells and RNA-Seq was performed on the HiSeq 2500 Rapid Run platform. (n=6) Differential gene-expression analysis was performed using DESeq2. Genes listed are significantly upregulated or downregulated with an adjusted p value < 0.5.