Table III.

Significant usage differences in term pregnancies (maternal blood versus cord blood)

	V Genes	D Genes	J Genes
TCR
Higher cord blood usage	TRBV6-5 (1.42), TRBV6-6 (1.62), TRBV10-3 (1.31)	TRBD2 (1.07)	TRBJ2-3 (1.22), TRBJ2-4 (1.42)
Higher maternal blood usage	TRBV7-8 (1.56), TRBV7-9 (1.33), TRBV18 (1.64)
IgD unmutated
Higher cord blood usage	IGHV6-1 (2.71)	IGHD1-7 (1.74), IGHD1-26 (1.57), IGHD6-6 (1.93), IGHD7-27 (9.08)	IGHJ1 (1.47), IGHJ2 (2.49), IGHJ3 (1.88), IGHJ4 (1.09)
Higher maternal blood usage	IGHV3-48 (1.37)	IGHD3-16 (1.29), IGHD4-17 (1.59), IGHD6-19 (1.43)	IGHJ5 (1.35), IGHJ6 (1.55)
IgD mutated
Higher cord blood usage		IGHD1-26 (1.55), IGHD6-6 (2.03), IGHD7-27 (5.97)	IGHJ2 (2.53), IGHJ3 (1.83)
Higher maternal blood usage		IGHD2-15 (1.45), IGHD5-24 (1.53), IGHD6-19 (1.42)	IGHJ5 (1.46), IGHJ6 (1.48)
IgM unmutated
Higher cord blood usage	IGHV6-1 (2.81)	IGHD1-7 (1.59), IGHD1-26 (1.43), IGHD6-6 (1.93), IGHD6-13 (1.34), IGHD7-27 (7.52)	IGHJ2 (2.15), IGHJ3 (1.69)
Higher maternal blood usage	IGHV1-46 (1.43), IGHV3-21 (1.23)	IGHD2-15 (1.62), IGHD3-10 (1.29), IGHD4-17 (1.56), IGHD6-9 (1.42)	IGHJ5 (1.52), IGHJ6 (1.54)
IgM mutated
Higher cord blood usage	IGHV1-3 (1.83)	IGHD1-26 (1.40), IGHD6-6 (2.07), IGHD6-13 (1.51), IGHD7-27 (5.68)	IGHJ2 (2.29), IGHJ3 (1.75)
Higher maternal blood usage	IGHV3-7 (1.60), IGHV3-48 (1.56), IGHV3-74 (1.92)	IGHD2-15 (1.56), IGHD3-16 (1.33), IGHD4-17 (1.49), IGHD6-19 (1.53), IGHD6-25 (1.62)	IGHJ4 (1.06), IGHJ5 (1.58), IGHJ6 (1.37)

Summary of term maternal versus neonate analysis for TCR-β and IgH V, D, and J gene segment usage. In pregnancies carried to term, a substantial number of genes had significantly higher usage in cord blood (compared with maternal blood) or in maternal blood (compared with cord blood), across TCR and various IgH isotypes. The usage difference in fold change is noted in parentheses next to each gene.