Figure 4. Adoptive transfer of pulmonary T cells does not protect against SARS-CoV-2 challenge.

A) Total T cells from naïve hACE2 mice or total T_eff from hACE2 immune animals were purified and 3.5x10⁵ cells were intratracheally transferred into naïve hACE2 animals or B) 1.4x10⁶ total T_eff from hACE2 immune animals were intravenously transferred into naïve hACE2 animals. One day later, mice were intranasally challenged with 10² PFU of SARS-CoV-2 and monitored for signs of illness. n=10 mice/group (A) or n=6-8 mice/group (B) and are combined from 2 independent experiments. Statistically significant differences in survival between groups was determined using a log-rank (Mantel-Cox) test and found not to be statistically significant.