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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Angew Chem Int Ed Engl. 2021 Oct 18;60(47):25034–25041. doi: 10.1002/anie.202110318

Table 1.

Encapsulated and unencapsulated GDH enzyme kinetic analyses.

Sample[a] Kcat [95% CI] [b] Kmapp [95% CI] [b] Vmax [95% CI] [b]
GDH 86.75 s−1 27.68 mM 0.77 μM/sec
[81.26, 92.71] [22.56, 33.98] [0.72, 0.82]
Disassembled 59.33 s−1 15.16 mM 0.53 μM/sec
GDH_T4GALA [55.99, 62.87] [12.81, 18.63] [0.50, 0.56]
Assembled 52.90 s−1 19.83 mM 0.47 μM/sec
GDH_T4GALA [47.28, 59.17] [13.63, 28.71] [0.42, 0.52]
[a]

Assays were conducted in triplicate and analyzed via non-linear regression.

[b]

Turnover number (kcat), apparent Michaelis-Menten constant (Kmapp) and maximum velocity (Vmax) were calculated via Michaelis-Menten curve fitting with least square method. CI, confidence interval; GDH, glucose dehydrogenase.