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. Author manuscript; available in PMC: 2022 Nov 8.
Published in final edited form as: Cancer Cell. 2021 Oct 7;39(11):1531–1547.e10. doi: 10.1016/j.ccell.2021.09.003

Figure 8: Subtype III CAFs are chemoattractant to immune cells.

Figure 8:

A. The status of tumor-infiltrating lymphocytes, based on CD8 staining, in EGFR+ NSCLC (n=10) according to functional subtypes of PDFs. Two-tailed Fisher’s exact test is used. B. The expression of indicated chemokines with chemoattractant properties for T-lymphocytes and monocytes in subtype III PDFs compared with subtypes I and II PDFs. Mean with 95% CI. * p < 0.05, two-tailed t test is used. C. Schematics of an ex vivo microfluidic assay to recapitulate the immune cell migration process. D. Representative images showing minimal (left) and substantial (right) immune cell migration in the microfluidic chip. E-F. Example images and summary of non-subtype III PDFs (subtypes I and II, n=4, example of a subtype I PDF is shown) and subtype III PDFs (n=4) in chemoattracting peripheral blood mononuclear cells (PBMC) (E) and peripheral blood CD8+ T cells (F) from two healthy donors. One of the representative interface areas is shown. Average level with 95%CI is shown. *, p < 0.05, one-tailed t-test is used. G. A graphic summary of the current study. See also Figure S7 and Table S5-6.