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Effect of relocation of the IRF-3 NES. The NES of IRF-3 was inserted in frame between GFP and the first amino acid of wt IRF-3 to create GFP–NES–IRF-3 (NES-wt) or it was inserted into the NES-deficient mutant (NES-IL/MM). The constructs were transfected into cells that were left untreated (a, c, e) or were infected with NDV (b, d, f), and fluorescent images are displayed. A diagrammatic representation of the constructs is shown.
