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Localization of a recombinant IRF-3–CBP fusion protein to the nucleus. HEC-1B cells were transfected with expression plasmids encoding either (a) GFP–IRF-3 (a.a. 1 to 327) or (b) GFP–IRF-3 (a.a. 1 to 327)–CBP. Fluorescence microscopic images display protein localization. Diagrammatic representation of the GFP–IRF-3–CBP construct is shown.
