Table 1.
Summary of the SEC-MALS and AUC sedimentation velocity and equilibrium experiments.
| BRD4 construct | State | Theoretical mass (Da) | Mass SEC-MALS (Da) | Equilibrium mass AUC (Da) | Average sedimentation coefficient (S) | f/f0* | Mass estimate |
|---|---|---|---|---|---|---|---|
| 1-530 | unphos. (bacteria) | 60,512.7 | 81,330 | 62,883 | 2.58 | 2.35 | 64,200 |
| insect | 60,569.7 | 86,970 | 68,124 | 3.18 | 2.10 | 79,500 | |
| CK2 phos. | 60,512.7 | 79,600 | 65,609 | 3.10 | 1.73 | 59,500 | |
| 1-579 | unphos. (bacteria) | 66,376.5 | 87,130 | 80,511 | 3.13 | 1.97 | 86,100 |
| insect | 66,433.5 | 129,500 | 91,765 | 4.42 | 2.40 | 152,000 | |
| CK2 phos. | 66,376.5 | 131,510 | 113,385 | 4.35 | 2.19 | 142,000 | |
| 1-722 | unphos. (bacteria) | 82,415.3 | 96,170 | 117,258 | 3.53 | 1.72 | 78,200 |
| insect | 82,472.3 | 147,200 | 145,923 | 5.65 | 1.58 | 123,000 | |
| CK2 phos. | 82,415.3 | 156,800 | 131,824 | 5.41 | 1.56 | 112,000 | |
| 7A CK2 phos. | 82,303.3 | 105,200 | – | – | – | – | |
| 6A CK2 phos. | 82,319.3 | 134,600 | – | – | – | – | |
| Δ506-530 insect | 80,404.9 | 91,200 | – | 3.71 | 1.76 | 80000 | |
| Δ506-530 λ-phosphatase | 80,404.9 | 81,750 | – | 2.95 | 2.41 | 82700 | |
| 1-722 λ-phosphatase | + iBET | 82,472.3 | 88,157 | – | – | – | – |
| + AZD5153 | 82,472.3 | 84,003 | – | – | – | – | |
| + 10 | 82,472.3 | 83,858 | – | – | – | – | |
| + 6 | 82,472.3 | 84,747 | – | – | – | – | |
| + 7 | 82,472.3 | 86,097 | – | – | – | – | |
| 1-722 insect | + iBET | 80,404.9 | 120,321 | – | – | – | – |
| + AZD5153 | 80,404.9 | 111,703 | – | – | – | – | |
| + 10 | 80,404.9 | 114,276 | – | – | – | – | |
| + 6 | 80,404.9 | 112,726 | – | – | – | – | |
| + 7 | 80,404.9 | 117,168 | – | – | – | – |
*f/f0= frictional coefficient.