Fig. 3.

C. glabrata-specific primer pairs Cg1, Cg2, and Cg3 detect small amounts of genomic DNA. (A) Amplification products obtained by endpoint PCR with primer pairs Cg1, Cg2, and Cg3 with decreasing amounts of genomic DNA as indicated (lanes 4 to 11). Lanes 1 and 12: molecular weight markers (MWM). Lanes 2 ( +) and 3 (-): positive and negative controls as described for Fig. 2B Amplification products with C. glabrata-specific primer pairs Cg1, Cg2, and Cg3 using as template mixes of 100 ng each of purified genomic DNA from C. albicans (Ca), C. tropicalis (Ct), C. parapsilosis (Cp), C. bracarensis (Cb), and S. cerevisiae (Sc), in addition to decreasing amounts of C. glabrata DNA. Lanes 1 and 8: MWM; lanes 2 ( +) and 3 (-): positive and negative controls as described for Fig. 2. Lanes 4 to 7: mixes of 100 ng each Ca, Ct, Cp, Cb, Sc genomic DNA plus 100 ng (lane 4), 10 ng (lane 5), 1 ng (lane 6), or 0 ng (lane 7) of C. glabrata genomic DNA