. 2021 Jul 31;52(4):1733–1744. doi: 10.1007/s42770-021-00584-2

Table 3.

Amplification and reaction conditions for the three C. glabrata-specific primer pairs and the ITS regions of the rDNA array

Primer pair name^a	Primer No	Annealing temperature	Final primer concentration in PCR reaction	Final dNTP concentration in PCR reaction	Expected sizes of amplicons^b (chromosome)
Cg1	242	59 °C	500 nM	30 µM	137 bp (chr. K) 139 bp (chr. E) 141 bp (chr. H)
Cg1	245	59 °C	500 nM	30 µM	137 bp (chr. K) 139 bp (chr. E) 141 bp (chr. H)
Cg2	245	67.1 °C	200 nM	30 µM	324 bp (chr. E) 348 bp (chr. K) 364 bp (chr. H)
Cg2	246	67.1 °C	200 nM	30 µM	324 bp (chr. E) 348 bp (chr. K) 364 bp (chr. H)
Cg3	243	63.4 °C	500 nM	30 µM	602 bp (chr. C left) 667 bp (chr. C right)^c
Cg3	244	63.4 °C	500 nM	30 µM	602 bp (chr. C left) 667 bp (chr. C right)^c
rDNA	ITS1	60 °C	500 nM	500 µM	878 bp (C. glabrata)^d 520 bp (C. parapsilosis) 524 bp (C. tropicalis) 535 bp (C. albicans)
rDNA	ITS4	60 °C	500 nM	500 µM

^aEach pair of primers consists of a forward and reverse primer which anneal to several positions in the C. glabrata genome and generate several amplicons

^bThe size of the amplicons is calculated in silico with the sequence of the strain CBS138

^cThe amplicon of the NE adjacent to EPA7 corresponds to the sequence from strain BG14 (GenBank Acc. No. AY646926.1)

^dThe amplicon expected for C. glabrata is 878 bp, while the amplicons for C. parapsiolisis, C. tropicalis, and C. albicans are very similar (520 bp, 524, and 535 bp respectively)[25]