Table. 1.
Isolates of dermatophytes used in the study with description, results of fluconazole sensitivity, and ERG gene expression analysis
| Host | Isolates | Type of infection/localization of skin lesions | Accession numbers of ITS sequences | MIC of fluconazole [µg/ml] | MIC interpretation* | ERG gene expression analysis** | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Exposed to fluconazole | Without exposure to fluconazole | |||||||||||
| ERG3 | ERG6 | ERG11 | ERG3 | ERG6 | ERG11 | |||||||
| Human | TV16 | Kerion celsi/head | MG251681 | 64 | R | 2.9 | 2.9 | 2.8 | 2.1 | 2.4 | 2 | |
| TV23 | Kerion celsi/head | MG251688 | 64 | R | 1.9 | 1.8 | 2.9 | 1.15 | 1.3 | 2.1 | ||
| TV28 | Kerion celsi/head | MG251693 | 128 | R | 2.2 | 2 | 2.9 | 1.9 | 1.5 | 2.1 | ||
| LL7 | Tinea unguium | MK369715 | 8 | R | 2.3 | 2.1 | 3 | 1.7 | 1.4 | 1.9 | ||
| TV35 | Tinea capitis | MT808415 | 32 | R | 2.4 | 2 | 3.1 | 1.9 | 1.6 | 2.2 | ||
| TV36 | Tinea capitis | MT808416 | 32 | R | 2.3 | 2.2 | 3.1 | 1.7 | 1.5 | 2.4 | ||
| TV46 | Tinea capitis | MT808417 | 8 | R | 2.2 | 2.4 | 3.9 | 1.6 | 1.3 | 2.6 | ||
| TV49 | Tinea capitis | MT808418 | 64 | R | 2.6 | 2 | 3.8 | 1.7 | 1.5 | 2.7 | ||
| TV51 | Tinea capitis | MT808419 | 16 | R | 2.4 | 2.9 | 3.8 | 1.6 | 1.3 | 2.9 | ||
| TV56 | Tinea capitis | MT808420 | 32 | R | 2.3 | 2.1 | 3.1 | 1.4 | 1.2 | 2.1 | ||
| Animals | Cattle | TV22 | Head, thorax | MG251687 | 128 | R | 2.5 | 2.7 | 3.2 | 1.3 | 1.5 | 2 |
| Cattle | TV24 | Head, thorax | MG251689 | 128 | R | 2.5 | 1.9 | 3.5 | 1.3 | 1.3 | 2.8 | |
| Sheep | TV25 | Head | MG251690 | 64 | R | 2.6 | 1.9 | 3.3 | 1.5 | 1.6 | 2.5 | |
| Sheep | TV26 | Head | MG251691 | 64 | R | 2.7 | 2.1 | 3.7 | 1.4 | 1.7 | 2.2 | |
| Cattle | TV27 | Head, thorax | MG251692 | 128 | R | 2.7 | 2.2 | 3.5 | 1.3 | 1.4 | 2.3 | |
| Cattle | TV8 | Head, thorax | MG251673 | 32 | R | 2.8 | 2.2 | 3.8 | 1.8 | 1.4 | 2.5 | |
| Cattle | TV1 | Head, thorax | MG251666 | 8 | R | 2.2 | 2 | 2.9 | 1.5 | 1.3 | 2 | |
| Sheep | TV20 | Head | MG251685 | 64 | R | 2.5 | 1.9 | 2.1 | 1.4 | 1.3 | 2.1 | |
| Llama | LL1 | Head, neck | MK369717 | 4 | R | 2.1 | 2.1 | 2.5 | 1.2 | 1.2 | 1.9 | |
| Llama | LL2 | Head, neck | MK369716 | 64 | R | 2.7 | 2.1 | 3 | 1.4 | 1.4 | 2.1 | |
| Alpaca | ALV12 | Neck | MN960481 | 2 | R | 2.4 | 2 | 2.9 | 1.5 | 1.5 | 2.3 | |
| Alpaca | ALV32 | Trunk | MN960484 | 64 | R | 2.7 | 1.9 | 3.1 | 1.6 | 1.5 | 2 | |
| Calves | TV38 | Multiple site | MT808618 | 32 | R | 2.7 | 2.6 | 3.4 | 1.3 | 1.8 | 1.9 | |
| Calves | TV45 | Multiple site | MT808619 | 64 | R | 2.8 | 1.9 | 3 | 1.4 | 1.5 | 1.9 | |
| Calves | TV50 | Head | MT808620 | 32 | R | 2.4 | 1.9 | 3.2 | 1.2 | 1.2 | 2.1 | |
| Cattle | TVS91 | Neck | MT818238 | 0.5 | S | 2 | 2 | 2.4 | 1.1 | 1.5 | 1.1 | |
| Cattle | TVS92 | Thorax | MT818239 | 0.5 | S | 1.7 | 1.5 | 2.3 | 1.2 | 1.2 | 1.9 | |
| Cattle | TVS93 | Head, neck | MT818240 | 0.25 | S | 1.9 | 1.45 | 2.9 | 1.4 | 0.9 | 0.9 | |
| Cattle | TVS94 | Thorax | MT818241 | 0.5 | S | 2.2 | 1.6 | 2.1 | 1.5 | 1.2 | 1.1 | |
| Cattle | TVS95 | Head | MT818242 | 0.5 | S | 2.9 | 1.6 | 2.2 | 2 | 1.3 | 1.2 | |
*R resistant, S susceptible; **geometric mean of the mRNA fold change for ERG genes normalized relative to GAPDH: standard deviation between replicates for each isolate did not exceed 5%