Table. 2.

Gene-specific primers used for real-time RT-PCR assays

Gene	Product	Primers (5′-3′)	Amplicon length (bp)
ERG3	Sterol C-5 desaturase	F: GACGGATACGTTCAGTCGCT R: GCCGTCGTGGATGAAGATTG	120
ERG6	Sterol C-24methyltransferase	F: CGAACACCTTTGATGCGGTG R: TTCATACACGCCAAACACGC	119
ERG11	Lanosterol 14-α demethylase	F: CTGACCCAGCCCATCAACAT R: TGGGGATGTTGCTCTTCACG	112
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	F: AACGGCTTCGGTCGTATTG R: TATTCGGCGTATTTGGTCTCA	110

F forward, R reverse