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. 2021 Nov 9;11:21995. doi: 10.1038/s41598-021-01483-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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FANTASTIC FOUR gene family evolution and expression in Ginkgo. (a) Maximum Likelihood (ML) analysis for FAF homologues across seed plants. Yellow stars indicate the three large scale duplication events detected. One before the diversification of angiosperms giving rise to the clades FAF1/2 and FAF3/4. And each clade has undergone one more duplication specific to Brassicaceae. (b–e) In situ hybridization for GibiFAF. (d) Pollen cone. (e) Leaf. e endothelium, i integument, l leaf, n nucellus, p, pollen. Scales: 75 μm (a,b); 100 μm (d,e).