Online-Only Table 6.
WGS Libraries were made from pooling the HCC1395 and HCC1395BL cell lines with various ratios (3:1, 1:1, 1:4, 1:9 and 1:19) to create mixtures.
| Sample ID | Sample(Mix ratio) | Biosample | Platform | Machine model | library Protocol(Iinput amount) | Total Reads | Total Reads After Trimming | Percent Total Reads after Trimming | Total Mapped Reads | Percent Total Mapped Reads (Trimmed) | Percent Non-duplicated Reads | Mean Coverage Depth | Mean Coverage Depth SD | Percent of Coverage > = 5X | Percent GC | Median Insert Size |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SPP_GT_0-1_1 | HCC1395:HCC1395BL (0:1) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,076,097,436 | 3,076,097,436 | 98.6 | 3,066,844,772 | 99.7 | 83.0 | 115.0 | 657.4 | 93.7 | 40.0 | 380 |
| SPP_GT_0-1_2 | HCC1395:HCC1395BL (0:1) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,001,832,062 | 3,001,832,062 | 99.9 | 2,993,554,635 | 99.7 | 86.1 | 112.0 | 627.8 | 93.7 | 40.0 | 391 |
| SPP_GT_0-1_3 | HCC1395:HCC1395BL (0:1) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 2,903,255,740 | 2,903,255,740 | 99.9 | 2,893,798,551 | 99.7 | 86.7 | 105.1 | 586.8 | 93.7 | 39.9 | 378 |
| SPP_GT_1-0_1 | HCC1395:HCC1395BL (1:0) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,077,848,110 | 3,077,848,110 | 98.9 | 3,069,341,612 | 99.7 | 76.4 | 117.9 | 475.3 | 93.1 | 40.2 | 368 |
| SPP_GT_1-0_2 | HCC1395:HCC1395BL (1:0) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,090,006,648 | 3,090,006,648 | 99.9 | 3,082,407,730 | 99.8 | 83.5 | 115.7 | 476.2 | 93.1 | 40.2 | 370 |
| SPP_GT_1-0_3 | HCC1395:HCC1395BL (1:0) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 2,962,991,404 | 2,962,991,404 | 99.9 | 2,954,244,873 | 99.7 | 87.8 | 107.5 | 448.9 | 93.1 | 40.1 | 376 |
| SPP_GT_1-1_1 | HCC1395:HCC1395BL (1:1) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,055,617,586 | 3,055,617,586 | 99.6 | 3,046,293,478 | 99.7 | 79.9 | 114.2 | 538.5 | 93.8 | 40.0 | 379 |
| SPP_GT_1-1_2 | HCC1395:HCC1395BL (1:1) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 2,972,612,956 | 2,972,612,956 | 100.0 | 2,963,334,376 | 99.7 | 78.4 | 107.8 | 532.6 | 93.8 | 39.8 | 383 |
| SPP_GT_1-1_3 | HCC1395:HCC1395BL (1:1) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,070,633,366 | 3,070,633,366 | 100.0 | 3,058,122,892 | 99.6 | 89.7 | 106.9 | 493.9 | 93.8 | 40.3 | 396 |
| SPP_GT_1-4_1 | HCC1395:HCC1395BL (1:4) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,031,054,048 | 3,031,054,048 | 97.5 | 3,018,769,715 | 99.6 | 74.4 | 114.9 | 609.9 | 93.8 | 40.0 | 369 |
| SPP_GT_1-4_2 | HCC1395:HCC1395BL (1:4) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 2,984,886,368 | 2,984,886,368 | 99.9 | 2,974,891,252 | 99.7 | 86.5 | 107.9 | 574.8 | 93.8 | 40.0 | 395 |
| SPP_GT_1-4_3 | HCC1395:HCC1395BL (1:4) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,076,330,144 | 3,076,330,144 | 98.5 | 3,065,365,108 | 99.6 | 70.8 | 111.9 | 576.8 | 93.8 | 40.1 | 392 |
| SPP_GT_1-9_1 | HCC1395:HCC1395BL (1:9) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,169,041,440 | 3,169,041,440 | 98.9 | 3,160,284,662 | 99.7 | 65.8 | 117.6 | 662.4 | 93.8 | 39.9 | 383 |
| SPP_GT_1-9_2 | HCC1395:HCC1395BL (1:9) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,001,203,286 | 3,001,203,286 | 99.9 | 2,993,060,766 | 99.7 | 84.9 | 109.1 | 605.5 | 93.8 | 39.9 | 384 |
| SPP_GT_1-9_3 | HCC1395:HCC1395BL (1:9) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,142,707,394 | 3,142,707,394 | 99.2 | 3,133,546,333 | 99.7 | 68.5 | 114.0 | 626.6 | 93.8 | 39.9 | 389 |
| SPP_GT_1-19_1 | HCC1395:HCC1395BL (1:19) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,113,584,442 | 3,113,584,442 | 98.3 | 3,104,340,674 | 99.7 | 63.0 | 115.4 | 663.9 | 93.7 | 39.8 | 381 |
| SPP_GT_1-19_2 | HCC1395:HCC1395BL (1:19) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 2,927,043,064 | 2,927,043,064 | 99.9 | 2,917,934,331 | 99.7 | 88.5 | 107.8 | 594.4 | 93.7 | 39.9 | 395 |
| SPP_GT_1-19_3 | HCC1395:HCC1395BL (1:19) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,044,009,076 | 3,044,009,076 | 99.9 | 3,033,242,220 | 99.6 | 90.6 | 109.1 | 579.2 | 93.8 | 40.2 | 397 |
| SPP_GT_3-1_1 | HCC1395:HCC1395BL (3:1) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,065,804,606 | 3,065,804,606 | 99.5 | 3,056,714,849 | 99.7 | 79.3 | 117.3 | 509.2 | 93.8 | 40.1 | 363 |
| SPP_GT_3-1_2 | HCC1395:HCC1395BL (3:1) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 3,052,239,564 | 3,052,239,564 | 100.0 | 3,042,838,917 | 99.7 | 66.1 | 112.7 | 523.1 | 93.8 | 39.8 | 376 |
| SPP_GT_3-1_3 | HCC1395:HCC1395BL (3:1) | Fresh cells | HiSeq | HiSeq 4000 | TruSeq DNA PCR Free (1000 ng) | 2,975,199,024 | 2,975,199,024 | 99.9 | 2,966,022,353 | 99.7 | 86.4 | 108.0 | 481.4 | 93.8 | 40.0 | 375 |
Libraries were prepared by using TruSeq DNA PCR Free (1000 ng) protocol and sequenced on Illumina HiSeq 4000.