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. 2021 Nov 9;11:21914. doi: 10.1038/s41598-021-01489-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro Validation. Tooth root volume assessment by CBCT segmentation with (A) OsiriX MD and (B) 3D Slicer software’s. (C) Illustrates volumetric analyses of the tooth root in Materialise MiniMagics software after scanning with Steinbichler COMET L3D laser equipment. (D) Illustrates volume estimation of the tooth root by water displacement method.