Skip to main content
. 2021 Nov 2;37(5):109943. doi: 10.1016/j.celrep.2021.109943

Figure 1.

Figure 1

Establishment of a degron to specifically degrade the ARID1A subunit of SWI/SNF complexes

(A) Western blot showing ARID1A depletion in mouse ESCs. mESCs expressing endogenous ARID1A protein homozygously tagged with mAID-GFP after the addition of 500 μM auxin for indicated time points, and ARID1A abundance in cells lines where ARID1A is knocked out or not tagged.

(B) The abundance of total cell proteins prior to and 2 h following growth in the presence of auxin indicates that ARID1A is specifically targeted for degradation via the degron.

(C) The abundance of SMARCA4-associated proteins determined by mass spectrometry is indicated as fold change of protein abundance prior to and 2 h following addition of auxin to cell media. Other than BAF-specific subunits ARID1A, DPF1, and DPF2 other subunits remain substantially associated with SMARCA4.