FIGURE 7.

α-SMA positive cells in old TNC-deficient and WT lungs. (A–D): flow cytometry quantification of α-SMA, desmin and vimentin in old female TNC KO and WT lung samples; proliferation status was evaluated using an EdU assay kit. N = 3 animals/genotype. Statistical analyses were made by Student’s t test; statistical significance was set at p < 0.05; *p < 0.05. (E,F): representative images of lung sections from TNC KO and WT old animals immunostained with anti-α-SMA antibody (dark gray; black arrows) and counter-stained with Nuclear Red. N = 3–5 animals/genotype. bv: blood vessel. (G,H): representative results of α-SMA protein detection in whole lung lysates from old TNC-deficient and WT animals by Western blot; quantification is shown in histogram; N = 6–7 animals/genotype. Cropped blots are displayed.