Figure 1.
Single-cell RNA-seq coupled to MS current characterization
(A) Schematic representation of the methodology used in this study.
(B) Frequency distribution of the inactivation time constants determined from mono- and bi-exponential fits of MS currents. Data were collected over 312 DRG neurons. Bin width was 0.5 ms for IRA and IIA, and 10 ms for ISA and IUltra-SA.
(C) Representative examples of the distinct types of MS currents with inactivation time course fitted with a mono-exponential function (black dashed line).
(D) Examples of MS currents fitted with bi-exponential functions (red dashed line). Black dashed lines show each mono-exponential component extracted from the fit.
For (C) and (D), top traces represent the mechanical probe displacement and bottom traces the recorded currents at −80 mV.
(E) Inactivation time-constant (τ) for all identified current components in scRNA-seq neurons with monophasic (circle) or biphasic (square) MS currents, n = 53 neurons from 16 mice.