Figure 2.

Cluster analysis of scRNA-seq neurons

(A) Visualization using t-SNE embedding of all cells colored by cluster identity. Triangles represent the scRNA-seq neurons of this study and circles the neurons from the study of Zeisel and collaborators. Main populations of neurons are surrounded by dashed lines. See Figure S1C for the heatmap of the expression of genes used for joint analysis.

(B) Heatmap of expression of marker genes in neurons grouped by population.

(C) Expression level of genes coding for thermosensitive and pruriceptive molecular sensor neurons grouped by population. NF, neurofilament; NP, non-peptidergic; TH, tyrosine hydroxylase; PEP, peptidergic.