Figure 5.

Tmem120a and Tmem150c do not contribute to DRG MS currents

(A) Representative images of fluorescent in situ hybridization for Tmem120a (right panel) in DRG neurons immuno-stained for peripherin and NF200 (left panel).

(B) Percentage of DRG neurons expressing Tmem120a mRNA in specified populations.

(C) Cross-sectional area distribution of Tmem120a mRNA-positive neurons. The top panel shows the percentage of Tmem120a positive neurons.

(D) Quantification of the prevalence of RA, IA, SA, and ultra-SA currents among DRG neurons electroporated with control, Piezo2, Tmem120a, or Tmem150c siRNAs. ^∗∗∗p < 0.001; χ² test.

(E) Prevalence of RA, IA, SA, and ultra-SA current components in siRNA-electroporated DRG neurons (top panels) and corresponding average maximal current amplitude (bottom panels). ^∗∗∗p < 0.001; ^∗p < 0.05; χ² test (top panels) and Kruskal-Wallis multiple comparison (bottom panels).

(F) Stacked histogram showing the prevalence of RA, IA, SA, and ultra-SA currents in IB4-positive neurons electroporated with control or Tmem120a siRNAs. χ² test shows no statistical difference. Inset: arrow shows a DRG neuron positive for IB4 (red) and siRNA transfection (green). Scale bar, 10 μm.

(G) Prevalence of RA, IA, SA, and ultra-SA current components in siRNA-electroporated IB4-positive neurons (top panels) and corresponding average maximal current amplitude (bottom panels). Mann-Whitney test shows no statistical difference.

For panels (D) and (F), neurons responding with biphasic MS currents were classified according to the MS current type contributing the most. NR, non-responders. For (E) and (G) top panels, solid and striped colors indicate neurons with monophasic or biphasic MS currents, respectively. For all panels, n numbers are indicated in brackets, and 3–6 mice were used for each siRNA condition. Error bars represent SEM.