Table 1.
Domestication requirement of GoldBricks and comparison with other techniques
| Domestication requirements of the restriction enzyme scheme | ||
|---|---|---|
| Goldbricks restriction enzymes and site length | ||
| Enzyme | Type | Restriction site length |
| NotI | Type II | 8 bp |
| MauBI | Type II | 8 bp |
| Esp3I | Type II-S | 6 bp |
| Eco31I | Type II-S | 6 bp |
| Comparison of domestication requirement in sample genomes | ||
| Strategy | Frequency of restriction sites in NtCp genome (150 kb) | Frequency of restriction sites in E.coli genome |
| BioBricks | 208 | 1448 |
| GoldBricks | 53 | 1581 |
| GoldenBraid 2.0 | 75 (100 for MoClo) | 6798 (3136 for MoClo) |
| Golden Gatea | 29 | 261 |
a
Of the four cloning methods listed here, Golden Gate cloning is not an endless cloning method.
NtCp, Nicotiana tabacum chloroplast.