TABLE 1.
Strains and plasmids used in this study
| Relevant characteristics | Source | |
|---|---|---|
| Plasmids | ||
| pUC118 | Ampr | Takara Bio |
| pK18mobsacB | Kmr, oriT (RP4), sacB, lacZα | Kvitko and Collmer (2011) |
| pUC18‐mini‐Tn7T‐Gm | Gmr | Choi et al. (2005) |
| pTNS2 | Helper plasmid carrying T7 transposase gene | Choi et al. (2005) |
| pMD20delta‐phcR | pMD20 derivative carrying a 1.4‐kb DNA fragment for phcR deletion, Ampr | This study |
| pdelta‐phcR | pK18mobsacB derivative carrying a 1.4‐kb DNA fragment for phcR deletion, Kmr | This study |
| pdelta‐phcQ | pK18mobsacB derivative carrying a 1.5‐kb DNA fragment for phcR deletion, Kmr | This study |
| pUC18‐mini‐Tn7T‐Gm‐phcQ | pUC18‐mini‐Tn7T‐Gm derivative carrying a 2.8‐kb fragment for phcR complementation, Gmr | This study |
| Escherichia coli strain | ||
| DH5α | recA1 endA1 gyrA96 thi‐1 hsdR17supE44 Δ(lac)U169(ϕ80lacΔM15) | Takara Bio |
| R. solanacearum strains | ||
| OE1‐1 | Wild‐type strain, phylotype I, race 1, biovar 4 | Kanda et al. (2003) |
| ΔphcB | phcB‐deletion mutant of OE1‐1 | Kai et al. (2015) |
| ΔphcA | phcA‐deletion mutant of OE1‐1 | Mori et al. (2016) |
| ΔphcK | phcK‐deletion mutant of OE1‐1 | Senuma et al. (2020) |
| ΔphcR | phcR‐deletion mutant of OE1‐1 | This study |
| ΔphcQ | phcQ‐deletion mutant of OE1‐1 | This study |
| phcQ‐comp | A transformant of ΔphcQ with pUC18‐mini‐Tn7T‐Gm‐phcQ containing native phcQ, Gmr | This study |
| lecM‐M | lecM::Tn EZ::TN <KAN‐2> mutant, Kmr | Mori et al. (2016) |