FIGURE 5.

siRNA targeting Rstps2 contributes to gene silencing and virulence inhibition. (a) The trehalose content of Rhizoctonia solani‐(TPS‐3‐2/6) was significantly decreased. Data are shown as the mean ± SE of triplicates. **p < .01, one‐way analysis of variance (ANOVA) and Tukey's test. (b) Relative gene expression of Rstps2 in standardized subcultured R. solani‐(TPS‐3‐2). G1–G5: generation 1 to generation 5. The data were normalized to the GAPDH transcript levels of GD‐118 and are shown as the mean ± SE of triplicates. Different labels represent statistical significance (p < .01), one‐way ANOVA and Tukey's test. (c) Each read length transcripts per million (TPM) sum of Rstps2‐derived specific siRNAs is the sum of all the TPM values of that read length. Data are shown as the mean ± SE of triplicates. Error bars represent SE (n = 3). *p < .05, Student's t test